RealThiol AM ester (RT-AM) provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.
RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage
Easily quantitate GSH levels in live cells using confocal microscopy/FACS
不用本金就能赚钱的方法Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve
不用本金就能赚钱的方法For calibration curve, see: RealThiol (RT) Glutathione (GSH) Detection Calibration Probe
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.