Mito RealThiol (MitoRT) provides ratiometric fluorescent readout for quantification of mitochondrial GSH in live mammalian cells.
不用本金就能赚钱的方法MitoRT is cell permeable and preferentially accumulated in mitochondria
不用本金就能赚钱的方法Conveniently compare mitochondrial GSH levels in live cells using confocal microscopy, and FACS
Signals from blue and green channels allow for deduction of mitochondrial GSH concentration, given an appropriate calibration curve
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.