These cell line express actin force probes, allowing for the measurement of forces of actin and actinin in living cells via simple anisotropy FRET ratio R to measure changes in FRET.
Integrate naturally into cellular microfilaments
不用本金就能赚钱的方法Report force in actin and the actin cross-linker actinin
Available as immortalized HEK293 or MDCK cell lines
AcpA - One actin molecule attached on each end of cpstFRET
cpA - One actin molecule attached to the C-terminal of cpstFRET, leaving the other end of the probe dangling free and the probe devoid of tension. (force-free control)
M-cpstFRET不用本金就能赚钱的方法 - Actinin with the sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin
C-cpstFRET - C-terminal–tagged actinin (force-free control)
Mechanical signaling plays many roles in cell physiology, including stem cell differentiation and reprogramming. The actin force probe, named actin–cpstFRET (circularly permuted stretch sensitive FRET)–actin (AcpA), consists of a dipole orientation-based FRET sensor called cpstFRET flanked with β-actin monomers. The sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs. Once epressed in cells, the actin probes incorporate into mainly into f-actin filaments and allow for the measurement of forces of actin and actin in in living cells via simple anisotropy FRET ratio R to measure changes in FRET.
不用本金就能赚钱的方法From the laboratory of Frederick Sachs, PhD, University at Buffalo.
|EBU022||Actin-cpstFRET-Actin (AcpA) HEK293 Cell Line||1 vial||In stock|
|EBU023||cpstFRET-Actin (cpA) HEK293 Cell Line||1 vial||In stock|
|EBU024||Actinin-M-cpstFRET (M-cpstFRET) HEK293 Cell Line||1 vial||In stock|
|EBU025||Actinin-C-cpstFRET (C-cpstFRET) HEK293 Cell Line||1 vial||In stock|
|EBU026||Actinin-M-cpstFRET (M-cpstFRET) MDCK Cell Line||1 vial||In stock|
|EBU027||Actinin-C-cpstFRET (C-cpstFRET) MDCK Cell Line||1 vial||In stock|